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Duck Egg White-Derived Peptide VSEE (Val-Ser-Glu-Glu) Regulates bone and lipid Metabolisms by Wnt/β-catenin Signaling Pathway and Intestinal Microbiota

Guo D1Liu W1Zhang X1Zhao M1Zhu B1Hou T1He H1.

Mol Nutr Food Res. 2019 Oct 31:e1900525. doi: 10.1002/mnfr.201900525. [Epub ahead of print]





Val-Ser-Glu-Glu (VSEE), identified from duck egg white peptides, has been proved to facilitate calcium absorption in previous study. Since prevention of osteoporosis is important, it might act as a potential cofactor in osteoporosis prevention. Therefore, the aim of this study was to investigate the regulation of VSEE on osteoporosis and abnormal lipid metabolisms.


MC3T3-E1 cell and OVX rat model were used to evaluate VSEE on regulation of bone and lipid metabolisms. Differentiation and matrix mineralization of preosteoblast were significantly increased by VSEE (P <0.05), which attributed to stimulating calcium influx, then to activating Wnt/β-catenin signaling pathway and regulating runt-related transcription factor 2 (Runx2) and osteoprotegerin (OPG). VSEE could cross Caco-2/HT-29 co-cultured monolayer via paracellular pathway and peptide transporter (PepT1), and could be detected in blood and maximum concentration was 122.84± 3.68 mg/L at 60 min. Additionally, VSEE was found to reverse bone loss and regulate dyslipidemia through Wnt/β-catenin signaling pathway in OVX rats. Firmicutes phylum, Veillonellaceae, Prevotellaceae and six genera in VSEE group were significantly different compared with Model group (P <0.05).


VSEE promotes bone growth and inhibit abnormal lipid metabolism in OVX model through the regulation of intestinal microbiota compositions and Wnt/β-catenin signal pathway.